微信公众号

官网二维码
中国癌症防治杂志

中国癌症防治杂志 ›› 2012, Vol. 4 ›› Issue (2): 136-139.doi: 10.3969/j.issn.1674-5671.2012.02.10

• 肝癌专栏 • 上一篇    下一篇

CCT亚基γ基因在AFB1诱发大鼠肝癌过程中表达的变化

  

  1. 广西肿瘤防治研究所实验研究部；广西医科大学第一附属医院肿瘤内科
  • 出版日期:2012-06-25 发布日期:2012-07-03
  • 通讯作者: 曹骥 E-mail:caojicn@yahoo.com.cn
  • 基金资助:

    广西自然科学基金资助项目（桂科基0832009； 桂科回0991017）

Expression of CCT subunit γ in aflatoxin B1-induced hepatocarcinogenesis in Wistar rats

  • Online:2012-06-25 Published:2012-07-03

PDF (PC)

404

赞

摘要： 目的 探讨AFB1诱发肝癌过程中CCT亚基γ基因蛋白的表达及其意义。方法  45只Wistar大鼠随机分为AFB1组和对照组，AFB1组以200μg/kg体重腹腔注射AFB1，第13周、第33周和第53周对大鼠进行肝活检，第73周处死全部动物并取肝组织。用免疫组化法检测AFB1诱发肝癌过程中不同时期CCT亚基γ基因蛋白的表达情况。结果 在第73周AFB1组CCT亚基γ基因蛋白表达水平显著高于对照组（P＜0.05）。同一组不同时段比较，随着诱癌的进行，CCT亚基γ基因蛋白表达上调，第73周AFB1组CCT亚基γ基因蛋白的表达水平不仅明显高于第13周，而且亦高于第33周（P＜0.01）。其余各组和同组不同时段之间CCT亚基γ基因蛋白的表达差异无统计学意义（P＞0.05）。AFB1组中发生肝癌的动物肝组织CCT亚基γ基因蛋白表达率为100%（10/10），显著高于同期对照组CCT亚基γ基因蛋白的表达率30%（3/10）（P＜0.05）。结论 AFB1诱癌过程中CCT亚基γ基因蛋白表达上调，CCT亚基γ基因蛋白有可能参与了肝细胞癌的发生和发展。

关键词: 肝肿瘤, CCT亚基γ基因蛋白, AFB1 , 大鼠肝癌, 蛋白表达

Abstract: Objective To investigate the protein expression and possible role of CCT subunit γ in hepatocarcinogenesis induced by aflatoxin B1（AFB1）in rats. Methods A total of 45 Wistar rats were randomly divided into a control group and an AFB1 group，which was injected intraperitoneally with 200 μg/kg AFB1.Liver biopsies were performed on all animals at weeks 13，33 and 53.Animals were sacrificed at week 73 and liver tissues were collected.Protein expression of CCT subunit γ was dynamically examined by immunohistochemistry during different phases of AFB1-induced hepatocarcinogenesis. Results The protein expression levels of the CCT subunit γ were significantly higher in HCC tissues of the AFB1 group（100%，10/10）than in tissues of the control group （30%，3/10）. The protein level gradually increased during development of hepatic carcinoma， with the level at week 73 significantly higher than at weeks 13 and 33（P<0.01）.At week 73，the level of CCT subunit γ was higher in the AFB1 group than in the control group （P<0.05）.The expression level of CCT subunit γ did not differ significantly between the two groups at other times（P>0.05）. Conclusion Overexpression of CCT subunit γ may be related to HCC carcinogenesis.

Key words:  Liver neoplasms, CCT subunit γ, Aflatoxin B1, Rat hepatocellular carcinoma；Protein expression